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C1354

Sigma-Aldrich

Monoclonal Anti-Caspase 11 antibody produced in rat

clone 17D9, purified from hybridoma cell culture

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About This Item

Numéro MDL:
MFCD02095975
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

17D9, monoclonal

Forme

buffered aqueous solution

Espèces réactives

mouse

Conditionnement

antibody small pack of 25 μL

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 5-10 μg/mL using mouse EL4 cell extracts.
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable

Isotype

IgG2a

Numéro d'accès UniProt

P70343

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Casp4(12363)

Description générale

Caspases are proteases having similar structure, substrate specificity and can be divided into three subfamilies based upon their amino acid sequence homology.Monoclonal Anti-Caspase 11 (rat IgG2a isotype) is derived from the 17D9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant p30 subunit of mouse caspase 11. Caspase 11 (also known as Ich-3) gene encodes two protein products of 38 and 43 kDa that belong to the Ced3/ICE family of cystein proteases. Caspase 11 is an “upstream” caspase with a long prodomain. Caspase 11 is identified in murine and shares homology with the human caspase 4 and 5.

Immunogène

recombinant p30 subunit of mouse caspase 11.

Application

Monoclonal Anti-Caspase 11 antibody can be used in immunohistochemistry and immunoprecipitation. It is also useful in immunoblotting

Actions biochimiques/physiologiques

Caspases are normally produced in the cell as inactive procaspases. Caspase 11 can induce apoptosis in cultured cells due to overexpression. Monoclonal antibodies reacting specifically with caspase 11 are useful tool for the study of the protease network, involved in development and regulation, governing the life and death of cells and tissues.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Caspase functions in cell death and disease
McIlwain DR, et al.
Cold Spring Harbor Perspectives in Biology, 5(4), a008656-a008656 (2013)
Xinyu Yang et al.
Immunity, 51(6), 983-996 (2019-12-15)
Excessive activation of the coagulation system leads to life-threatening disseminated intravascular coagulation (DIC). Here, we examined the mechanisms underlying the activation of coagulation by lipopolysaccharide (LPS), the major cell-wall component of Gram-negative bacteria. We found that caspase-11, a cytosolic LPS receptor, activated the
Qi Zhou et al.
Frontiers in bioengineering and biotechnology, 10, 823933-823933 (2022-03-31)
The communication between macrophages and tendon cells plays a critical role in regulating the tendon-healing process. However, the potential mechanisms through which macrophages can control peritendinous fibrosis are unknown. Our data showed a strong pro-inflammatory phenotype of macrophages after a
Chuang Yuan et al.
Cell death & disease, 13(8), 722-722 (2022-08-19)
Sepsis is a life-threatening syndrome with disturbed host responses to severe infections, accounting for the majority of death in hospitalized patients. However, effective medicines are currently scant in clinics due to the poor understanding of the exact underlying mechanism. We
Caspase-11: the driving factor for noncanonical inflammasomes
Vigano E and Mortellaro A
European Journal of Immunology, 43(9), 2240-2245 (2013)
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