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SAE0091

Sigma-Aldrich

Lysostaphine from Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

Synonyme(s) :

Glycyl-glycine endopeptidase

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About This Item

Numéro CAS:
9011-93-2
Numéro de classification (Commission des enzymes):
3.4.24.75 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131556
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54

product name

Lysostaphine from Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

Source biologique

microbial (Staphylococcus staphylotyticus)

Forme

lyophilized powder

Activité spécifique

≥500 units/mg protein

Poids mol.

27 kDa

Caractéristiques

DNA free

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Application

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Actions biochimiques/physiologiques

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
La lysostaphine est une endopeptidase à zinc d'un poids moléculaire de 25 kDa environ. Comme la lysostaphine clive les réticulations de type polyglycine dans la couche de peptidoglycane de la paroi cellulaire de Staphylococcus, elle s'est révélée utile pour la lyse cellulaire et comme potentiel agent thérapeutique anti-microbien.
pH optimal en termes d'activité : ~7,5

Définition de l'unité

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Forme physique

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Ronald M Galiwango et al.
Microbiome, 10(1), 7-7 (2022-01-20)
Coronal sulcus (CS) anaerobe abundance and IL-8 levels are linked to HIV acquisition, and are dramatically reduced after penile circumcision (PC). The distal urethra may be the site of some HIV acquisition before PC, and presumably most acquisition post PC. We
Kojiro Tsujihana et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2116027119-e2116027119 (2022-06-16)
The epidermis is the outermost layer of the skin and the body's primary barrier to external pathogens; however, the early epidermal immune response remains to be mechanistically understood. We show that the chemokine CXCL14, produced by epidermal keratinocytes, exhibits robust
Caroline Meyer Olesen et al.
Microorganisms, 9(7) (2021-08-08)
Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin
Astrid Haaskjold Lossius et al.
Dermatology (Basel, Switzerland), 238(1), 109-120 (2021-04-23)
The pathophysiology in atopic dermatitis (AD) is not fully understood, but immune dysfunction, skin barrier defects, and alterations of the skin microbiota are thought to play important roles. AD skin is frequently colonized with Staphylococcus aureus (S. aureus) and microbial
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
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