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A9275

Sigma-Aldrich

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

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About This Item

Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

egg white

Niveau de qualité

Gamme de produits

BioUltra

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized powder

Activité spécifique

≥10 units/mg protein (E1%/280)

Poids mol.

glycoprotein 66 kDa
subunit 16 kDa

Température de stockage

2-8°C

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Description générale

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. The product has also been used as a blocking agent in ELISA applications.

Définition de l'unité

One unit will bind 1.0 μg of d-biotin.

Notes préparatoires

Affinity purified

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Q Wang et al.
The American journal of physiology, 275(5 Pt 2), R1584-R1592 (1998-10-29)
We examined the effect of endotoxemia in mice on protein and mRNA levels for the acute phase proteins complement C3 and serum amyloid A (SAA) in jejunal mucosa. Endotoxemia was induced in mice by the subcutaneous injection of 250 microg
Lee B Sims et al.
Journal of nanobiotechnology, 15(1), 67-67 (2017-10-07)
Advanced stage cancer treatments are often invasive and painful-typically comprised of surgery, chemotherapy, and/or radiation treatment. Low transport efficiency during systemic chemotherapy may require high chemotherapeutic doses to effectively target cancerous tissue, resulting in systemic toxicity. Nanotherapeutic platforms have been
Roland Hager et al.
Scientific reports, 7, 42075-42075 (2017-02-16)
Arrays of individual molecules can combine the advantages of microarrays and single-molecule studies. They miniaturize assays to reduce sample and reagent consumption and increase throughput, and additionally uncover static and dynamic heterogeneity usually masked in molecular ensembles. However, realizing single-DNA
Lee B Sims et al.
Investigative ophthalmology & visual science, 60(5), 1696-1705 (2019-04-23)
The goal of this work was to design and assess the ability of unmodified and surface-modified poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) to enhance cell association, provide efficacy in retinoblastoma cells, and overcome current administration challenges, including hydrolysis and precipitation, of
Jana Svobodová et al.
PeerJ, 9, e12401-e12401 (2021-11-27)
Microbiome formation and assemblage are essential processes influencing proper embryonal and early-life development in neonates. In birds, transmission of microbes from the outer environment into the egg's interior has been found to shape embryo viability and hatchling phenotype. However, microbial

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