A7646

Apyrase from potatoes

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

• Synonyme(s) : Adénosine 5′-diphosphatase, Adénosine 5′-triphosphatase
• Numéro CAS: 9000-95-7
• Numéro de classification (Commission des enzymes): 3.6.1.5 (BRENDA, IUBMB)
• Numéro CE : 232-569-8
• Numéro MDL: MFCD00130542
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: potato
• Niveau de qualité: 200
• Type: Grade III
• Forme: lyophilized powder (partially purified)
• ATPase activity: ≥60 units/mg protein
• Poids mol.: ~49 kDa by gel filtration
• Composition: Protein, 30-60%
• Solubilité: H₂O: soluble 10 mg/mL, slightly hazy
• Application(s): diagnostic assay manufacturing
• Activité étrangère: Acid Phosphatase ≤5% of base activity
• Température de stockage: −20°C

Description

Application

Au moins deux isoenzymes sont présentes dans diverses souches de S. tuberosum : l′une avec un rapport ATPase/ADPase élevé (∼ 10) et l′autre avec un rapport faible (∼ 1).
Réaction : ATP → ADP+P_i → AMP+2P_i.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.

Actions biochimiques/physiologiques

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Conditionnement

Sold on the basis of ATPase units

Autres remarques

Mixture of high and low ratio isoenzymes.

Qualité

Enthält Säurephosphatase.

Définition de l'unité

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)