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06966

Sigma-Aldrich

Atto 655-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonyme(s) :

Biotin-Atto 655

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About This Item

Code UNSPSC :
12352116
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Pureté

≥90% (HPCE)

Forme

powder

Fabricant/nom de marque

ATTO-TEC GmbH

Transmittance

254 nm
655 nm

Solubilité

DMF: soluble
DMSO: soluble
acetonitrile: soluble

Fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Application

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Chemistry (Weinheim an der Bergstrasse, Germany), 17(50), 14170-14177 (2011-11-16)
The molecular interactions of the glycopeptide antibiotic vancomycin (Van) with bacterial cell wall analogues N,N'-diacetyl-L-Lys-D-Ala-D-Ala (Ac(2) KdAdA) and N,N'-diacetyl-L-Lys-D-Ala-D-Lac (Ac(2) KdAdL) were investigated in neat water, phosphate buffer and HEPES buffer by using fluorescence correlation spectroscopy (FCS) and molecular dynamics
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Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 696-703 (2011-01-29)
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