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Y1500

Sigma-Aldrich

YPD Agar

Synonyme(s) :

Yeast Extract Peptone Dextrose Agar, YEPD Agar

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About This Item

Code UNSPSC :
50405901
eCl@ss :
42040102
Nomenclature NACRES :
NA.75

Forme

powder

Niveau de qualité

200

Composition

Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

Application(s)

food and beverages

Adéquation

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Catégories apparentées

Description générale

YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.

Application

YPD agar has been used as a culture media:
  • to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
  • for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
  • for Zymoseptori triciti strains for virulence studies

Notes préparatoires

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000400585
0000357358
0000379714
0000338554
0000274275

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Protocoles

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