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Key Documents

90903

Sigma-Aldrich

Schaeffer and Fulton Spore Stain Solution A

for microscopy

Synonyme(s) :

Malachite Green Solution according to Schaeffer and Fulton

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12171500
Nomenclature NACRES :
MA.02

Qualité

for microscopy

Gamme de produits

BioChemika

Forme

solution

Durée de conservation

limited shelf life, expiry date on the label

Technique(s)

microbe id | staining: suitable

Adéquation

spore forming microorganisms

InChI

1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2

Clé InChI

CNYGFPPAGUCRIC-UHFFFAOYSA-L

Description générale

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin (Schaeffer & Fulton`s Spore Stain B, Cat. No. 39955). The components and the method are also available as kit (Cat. No. 04551).

Composants

Composition:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardCorrosionEnvironment

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Consulter la Bibliothèque de documents

Xiaofang Jia et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(42), 13494-13500 (2012-09-05)
This work describes a novel strategy for the highly sensitive and selective detection of cysteine (Cys) and glutathione (GSH) based on the Hg(2+)-AGRO100-malachite green (MG) complex system. The dye MG, which has a very low quantum yield in aqueous solution
Jia Zeng et al.
Biophysical journal, 104(1), 139-145 (2013-01-22)
It is shown that the nonlinear optical phenomenon known as second-harmonic generation can be used for label-free, time-resolved study of the transport of molecules through living cell membranes. The adsorption and transport of a 300-Da molecular-mass hydrophobic ion at the
Jagadeeswaran Chandrasekar et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(14), 5315-5320 (2013-03-20)
Catalytic DNA sequences (deoxyribozymes, DNA enzymes, or DNAzymes) have been identified by in vitro selection for various catalytic activities. Expanding the limits of DNA catalysis is an important fundamental objective and may facilitate practical utility of catalysts that can be
Liping Wen et al.
Advanced materials (Deerfield Beach, Fla.), 24(46), 6193-6198 (2012-09-29)
A highly efficient and perfectly reversible ionic gate that can be activated by pH or UV light is demonstrated. Switching between the OFF state and the ON state is mainly dependent on the surface charge transition brought about by a
Amanda R Sherwood et al.
Analytical biochemistry, 435(1), 54-56 (2012-12-04)
With the recent discovery of a unique class of dual-specificity phosphatases that dephosphorylate glucans, we report an in vitro assay tailored for the detection of phosphatase activity against phosphorylated glucans. We demonstrate that, in contrast to a general phosphatase assay

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