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114413

Sigma-Aldrich

Bromothymol Blue

ACS reagent, Dye content 95 %

Synonyme(s) :

3′,3″-Dibromothymolsulfonphthalein

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About This Item

Formule empirique (notation de Hill):
C27H28Br2O5S
Numéro CAS:
76-59-5
Poids moléculaire :
624.38
Numéro Beilstein :
373934
Numéro CE :
200-971-2
Numéro MDL:
MFCD00005872
Code UNSPSC :
12171500
ID de substance PubChem :
24847171
Nomenclature NACRES :
NA.47

Qualité

ACS reagent

Niveau de qualité

200

Forme

powder

Composition

Dye content, 95%

Couleur

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

Pf

200-202 °C ((392 - 396 °F) - lit.)
200-202 °C (lit.)

λmax

420 nm

ε (coefficient d'extinction)

≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol

Température de stockage

room temp

Chaîne SMILES 

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

Clé InChI

NUHCTOLBWMJMLX-UHFFFAOYSA-N

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Description générale

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000352443
0000352443
SHBQ9942
0000293123
0000234446

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Reza Amin et al.
Micromachines, 11(6) (2020-07-01)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic
Mariana A Sanchez et al.
Analytica chimica acta, 694(1-2), 95-99 (2011-05-14)
Liquid-liquid microextraction without phase segmentation was implemented in a multicommuted flow system for determination of the anti-hypertensive diltiazem. The procedure was based on ion pair formation between the drug and the dye bromothymol blue at pH 3.5. The detection was
Kristina Jurcic et al.
Journal of chromatography. A, 1134(1-2), 317-325 (2006-10-07)
An effective sample preconcentration technique for proteins and peptides was recently developed using capillary electrophoresis (CE) with discontinuous buffers [C.A. Nesbitt, J.T.-M. Lo, K.K.-C. Yeung, J. Chromatogr. A 1073 (2005) 175]. Two buffers of different pH created a junction to
Bambang Kuswandi et al.
Talanta, 74(4), 613-618 (2008-03-29)
An optical fiber biosensor consisting of acetylcholinesterase (AChE) and bromothymol blue (BTB) doped sol-gel film was employed to detect organophosphate pesticide chlorpyrifos. The main advantage of this optical biosensor is the use of a single sol-gel film with immobilized AChE
K R Klein et al.
Journal of clinical microbiology, 47(11), 3669-3672 (2009-10-02)
Cryptococcus neoformans and Cryptococcus gattii are closely related pathogenic fungi. Cryptococcus neoformans is ecologically widespread and affects primarily immunocompromised patients, while C. gattii is traditionally found in tropical climates and has been reported to cause disease in immunocompetent patients. l-Canavanine
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