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RSTREP-RO

Roche

Streptavidin, recombinant

liquid, ≥17 units/mg protein, pH range 5.0-9.0

Synonyme(s) :

streptavidin

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About This Item

Code UNSPSC :
12352202

Source biologique

Streptomyces sp. ( Streptomyces avidinii)

Niveau de qualité

100

Produit recombinant

expressed in E. coli

Forme

liquid

Activité spécifique

≥17 units/mg protein

Poids mol.

~52 kDa

Conditionnement

pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)

Fabricant/nom de marque

Roche

Concentration

70-90%

Technique(s)

protein array: suitable
protein purification: suitable

Impuretés

6-7% water

Plage de pH

5.0-9.0

pl 

7.4-7.7 (two main bands)

Solubilité

water: 10 mg/L (mA405=100)

Adéquation

suitable for immunological tests

Numéro d'accès UniProt

P22629

Conditions d'expédition

cooler

Température de stockage

-10 to -25°C

Description générale

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Séquence

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Définition de l'unité

One unit binds 1 μg D-biotin at pH 8.9.

Forme physique

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.
Dehlinger D, et al.
Journal of the Association for Laboratory Automation, 5, 267-276 (2007)
Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity
Jouybari RM et al.
Reports of Biochemistry and Molecular Biology, 6(2), 178-185 (2018)
John E G McCarthy et al.
Methods in enzymology, 430, 247-264 (2007-10-05)
A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been
Michael Weber et al.
Nature biotechnology, 41(4), 569-576 (2022-11-08)
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the
Michael Weber et al.
Nature photonics, 15(5), 361-366 (2021-05-07)
We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point
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