11363743001
Roche
Nutridoma™-CS
suitable for hybridoma, solution, pkg of 10 mL (50x)
Synonyme(s) :
serum replacements
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About This Item
Code UNSPSC :
12352207
Produits recommandés
Stérilité
sterile; 0.2 μm filtered
Niveau de qualité
Forme
solution
Conditionnement
pkg of 10 mL (50x)
Fabricant/nom de marque
Roche
Concentration
50 ×
Technique(s)
cell culture | hybridoma: suitable
Impuretés
mycoplasma, tested
100 EU/mL endotoxin (LAL test)
Solubilité
water: miscible
Température de stockage
−20°C
Description générale
Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.
Application
Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.
Définition de l'unité
Endotoxin (LAL): 100 EU/ml, mycoplasma tested
Forme physique
Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.
Notes préparatoires
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Informations légales
Nutridoma is a trademark of Roche
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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Les clients ont également consulté
Debora Steiner et al.
Nature biotechnology, 28(4), 361-364 (2010-03-31)
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Cellular reprogramming, 16(3), 185-195 (2014-05-09)
Four normal-karyotype human embryonic stem cell (hESC) lines were generated using the same protocol and maintained under identical conditions. Despite these precautions, gene expression patterns were found to be dissimilar among the four lines. The observed differences were typical of
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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