10110434001

Xanthine Oxidase (XOD)

from cow milk

• Synonyme(s) : XOD
• Numéro de classification (Commission des enzymes): 1.1.3.22 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: bovine milk
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• Conditionnement: pkg of 1 mL (20 U)
• Fabricant/nom de marque: Roche
• pH optimal: 8.5-9.0
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

Application

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

Actions biochimiques/physiologiques

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Qualité

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Séquence

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Définition de l'unité

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Forme physique

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Notes préparatoires

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash