10106399001

GTP

>90% (HPLC), crystals, pkg of 250 mg

• Synonyme(s) : Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na₂
• Formule empirique (notation de Hill): C₁₀H₁₆N₅O₁₄P₃ · xNa⁺ · yH₂O
• Numéro CAS: 36051-31-7
• Poids moléculaire : 523.18 (anhydrous free acid basis)
• Numéro Beilstein : 4113439
• Numéro MDL: MFCD00149227
• Code UNSPSC : 41106305
• ID de substance PubChem : 329748894

Propriétés

• Description: Disodium salt
• Niveau de qualité: 100
• Pureté: >90% (HPLC)
• Forme: crystals
• Poids mol.: M_r 523.2 (GTP); M_r 567.1 (GTP-Na₂)
• Conditionnement: pkg of 250 mg
• Fabricant/nom de marque: Roche
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Chaîne SMILES: O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1
• Clé InChI: HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

Description

Description générale

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.

GTP is supplied as the disodium salt in crystal form.

Application

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

Qualité

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na⁺, 8% H2O

Notes préparatoires

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Stockage et stabilité

Store at 2 to 8 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable