MABC612

Anti-Procadherin FAT1 Antibody, clone C257

clone C257, from mouse

• Synonyme(s) : Cadherin family member 7, Cadherin-related tumor suppressor homolog, Protein fat homolog
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: C257, monoclonal
• Espèces réactives: human
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_005236
• Numéro d'accès UniProt: Q14517
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FAT1(2195)

Description

Description générale

Procadherin FAT1 protein is a cell membrane bound protein that also has an active nuclear-translocation component that can alter specific gene expression and cell proliferation. Procadherin FAT1 is important in cell polarization and directed cell migration and also modulates cell-cell contact interactions. Procadherin FAT1 is expressed in epithelial, endothelial and smooth muscle cells and plays roles in their response to injury and is often highly induced after arterial injury and effects vascular smooth muscle cell growth and migration. Procadherin FAT1 is also important in cancer where it appears to function as both an oncogene and a tumor suppressor depending upon the cell context. Also recent research has shown that soluble FAT1 can be a novel biomarker candidate for such cancers as pancreatic cancer.

Immunogène

Recombinant protein corresponding to Human FAT1.

Application

Research Category
Apoptosis & Cancer

This Anti-Procadherin FAT1 Antibody, clone C257 is validated for use in Western Blotting and Immunoprecipitation for the detection of Procadherin FAT1.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MV3 cell lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MDA-MB-231 and MDA-MB-468 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Procadherin FAT1 in C257 cell lysate. (Courtesy of Dr. R. Thorne, U of Newcastle, AU.)

Qualité

Evaluated by Western Blotting in FAT1 transfected NIH-3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Procadherin FAT1 in 10 µg of FAT1 transfected NIH-3T3 cell lysate.

Description de la cible

>260 kDa observed. Procadherin FAT1 is an extremely large cell surface receptor with a calculated MW of 506 kDa and it′s highly glycosolated. It may be difficult to resolve the protein by western blot. An uncharacterized band at 50 kDa and other lower MWs may be observed in some lysates.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable