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AP187B

Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed

1.1 mg/mL, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

biotin conjugate

Forme d'anticorps

F(ab′)2 fragment of affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

rabbit

Fabricant/nom de marque

Chemicon®

Concentration

1.1 mg/mL

Technique(s)

ELISA: suitable
western blot: suitable

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Spécificité

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

Application

Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications:

1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin

1:10,000-1:100,000 for western blotting with chemiluminescent substrates.

1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.

1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry

Optimal working dilutions must be determined by the end user.
This Goat anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Rabbit IgG.

Forme physique

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% Sodium Azide

RECONSTITUTION:

Reconstitute with 1.5 mL sterile distilled water.

Stockage et stabilité

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C with or without aliquotting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Jing Ying Ma et al.
Journal of asthma and allergy, 1, 31-44 (2008-01-01)
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Kelly Koral et al.
The American journal of pathology, 192(6), 887-903 (2022-04-08)
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Expression of hepatocyte epidermal growth factor receptor, FAS and glypican 3 in EpCAM-positive regenerative clusters of hepatocytes, cholangiocytes, and progenitor cells in human liver failure.
Hattoum, A; Rubin, E; Orr, A; Michalopoulos, GK
Human Pathology null
Jun Maeda et al.
Brain communications, 3(1), fcab011-fcab011 (2021-03-02)
Microglia are the resident phagocytes of the central nervous system, and microglial activation is considered to play an important role in the pathogenesis of neurodegenerative diseases. Recent studies with single-cell RNA analysis of CNS cells in Alzheimer's disease and diverse
Satyanarayana Medicherla et al.
The Journal of pharmacology and experimental therapeutics, 318(1), 99-107 (2006-04-11)
Mitogen-activated protein kinases (MAPKs) and heat shock proteins (HSPs) are ubiquitous proteins that function within T cells in both normal and stress-related pathophysiological states, including type 1 diabetes. The nonobese diabetic (NOD) mouse spontaneously develops T cell-mediated autoimmune pancreatic beta

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