ABS2208

Anti-GPR161

• Synonyme(s) : G-protein coupled receptor 161
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Poids mol.: calculated mol wt 60.3 kDa; observed mol wt ~60. kDa
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse
• Conditionnement: antibody small pack of 100
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_001074595
• Numéro d'accès UniProt: B2RPY5
• Température de stockage: -10 to -25°C
• Informations sur le gène: mouse ... Gpr161(240888)

Description

Spécificité

This rabbit polyclonal antibody detects G-protein coupled receptor 161 (GPR161). It targets an epitope within 28 amino acids from the C-terminal, cytoplasmic domain.

Immunogène

KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal, cytoplasmic domain of mouse G-protein coupled receptor 161 (GPR161).

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cell transiently overexpressing human GPR161

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GPR161 in lysate from HEK293T cell transiently overexpressing human GPR161.

Tested Applications

Immunofluorescence Analysis: A representative lot detected GPR161 in Immunofluorescence application (Shimada, H., et. al. (2017). Cell Rep. 20(2):384-396).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GPR161 in Immunoprecipitation application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

Immunocytochemistry Analysis: A representative lot detected GPR161 in Immunocytochemistry application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

G-protein coupled receptor 161 (UniProt: B2RPY5; also known as GPR161) is encoded by the Gpr161 (also known as Gm208) gene (Gene ID: 240888) in murine species. GPR161 is a multi-pass membrane glycoprotein of the GPCR family with seven transmembrane domains. It localizes to the ciliary membrane and is also detected in the recycling endocytic compartment. It is ubiquitously expressed from 8.5 dpc and is mostly concentrated in the developing nervous system at later stages. By 10.5 dpc, it is mainly expressed in the neural tube. At later embryonic stages it is predominantly expressed in the brain, spinal cord, and dorsal ganglia. GPR161 functions as a negative regulator of sonic hedgehog (Shh) signaling, which promotes the processing of Gli3 into its repressive form (Gli3R) during neural tube development via cAMP-protein kinase A signaling. In the presence of Shh, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity, and allowing activation of the Shh signaling. Gpr161 knockout mice display increased Shh signaling and exhibit embryonic lethality by 10.5 dpc. GPR161 is overexpressed in triple-negative breast cancer and serves is a regulator of mammary epithelial cell proliferation and invasion in a mTOR-dependent manner. Knockdown of GPR161 is reported to impair proliferation of human basal breast cancer cell lines and its overexpression increases cell proliferation, migration, and intracellular accumulation of E-cadherin. Three isoforms of GPR161 have been described that are produced by alternative splicing. (Ref.: Hwang, S-H., et al. (2021). eLife 10:e67121; Mukhopadhyay, S., et al. (2013). Cell 152(1-2); 210-223; Feigin, ME., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(11); 4191-4196).

Forme physique

Purified rabbit polyclonal antibody in PBS without azide.

Reconstitution

0.5 mg/mL Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable