506119
PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb
liquid, Calbiochem®
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
rat, mouse, canine, human
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
phosphorylation (pThr180/pTyr182)
Informations sur le gène
human ... MAPK14(1432)
Description générale
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
This PhosphoDetect Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .
Immunogène
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
Application
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Immunocytochemistry (see comments)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In Dulbecco′s PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Remarque sur l'analyse
Positive Control
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
Autres remarques
Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Marinissen, M.J., et al. 2001. Genes Dev.15, 535.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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