234409

Complement C9-Depleted Serum, Human

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Forme: liquid
• Ne contient pas: preservative
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Human serum depleted of complement component C9 by affinity chromatography.

Human serum depleted of complement component C9 by affinity chromatography. C9-depleted serum, sterile-filtered through a 0.22 µm filter. No C9 detected by Ouchterlony using various dilutions of goat anti-human C9 antibody.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

Classical Pathway Activity: One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.

Forme physique

In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.

Notes préparatoires

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable