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Principaux documents

P6001

Sigma-Aldrich

p-Phenylenediamine

98% (GC)

Synonyme(s) :

1,4-Benzenediamine, 1,4-Diaminobenzene, 1,4-Phenylenediamine

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About This Item

Formule linéaire :
C6H4(NH2)2
Numéro CAS:
Poids moléculaire :
108.14
Beilstein:
742029
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
ID de substance PubChem :
Nomenclature NACRES :
NA.83
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Densité de vapeur

3.7 (vs air)

Niveau de qualité

Pression de vapeur

1.08 mmHg ( 100 °C)

Description

anti-fade reagent

Essai

98% (GC)

pb

267 °C (lit.)

Pf

138-143 °C (lit.)

Chaîne SMILES 

Nc1ccc(N)cc1

InChI

1S/C6H8N2/c7-5-1-2-6(8)4-3-5/h1-4H,7-8H2

Clé InChI

CBCKQZAAMUWICA-UHFFFAOYSA-N

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Application

p-Phenylenediamine has been used:
  • to evaluate its sensitization potential in vitro in langerhans-like dendritic cells and autologous T cells[1]
  • to treat the dorsal sides of the ears of mice, to study its systemic immunological effects[2]
  • in glycerol to coverslip coronal sections in the attempt to count Aβ plaques[3]

Anti-fade reagent is added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.

Actions biochimiques/physiologiques

p-Phenylenediamine causes allergic reactions with skin.[4] It is widely used in hair dyes. It eventually forms Bandrowski′s base, which is found to be the primary cause for allergy.[5] p-Phenylenediamine exposure results in dermatitis, urticaria and anaphylaxis.[6] It acts as an electron donor and is known to reduce cytochrome c.

Attention

Darkens in storage.

Pictogrammes

Skull and crossbonesHealth hazardEnvironment

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 1 Oral

Organes cibles

Kidney,Heart,Musculo-skeletal system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

230.0 °F - closed cup

Point d'éclair (°C)

110 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ying-Zhi Xu et al.
NAR cancer, 2(4), zcaa029-zcaa029 (2020-10-24)
Diffuse large B-cell lymphoma (DLBCL) is a molecularly heterogeneous group of malignancies with frequent genetic abnormalities. G-quadruplex (G4) DNA structures may facilitate this genomic instability through association with activation-induced cytidine deaminase (AID), an antibody diversification enzyme implicated in mutation of
p-Phenylenediamine
DeLeo VA
Dermatitis : Contact, Atopic, Occupational, Drug : Official Journal of the American Contact Dermatitis Society, North American Contact Dermatitis Group, 17(2), 53-55 (2006)
Identification and quantification of para-phenylenediamine in a temporary black henna tattoo
Brancaccio RR, et al.
American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society, 13(1), 15-18 (2002)
In vitro evaluation of the sensitization potential of weak contact allergens using Langerhans-like dendritic cells and autologous T cells
Rougier N, et al.
Toxicology, 145(1), 73-82 (2000)
p-Phenylenediamine allergy: the role of Bandrowski's base
White JML, et al.
Clinical and Experimental Allergy, 36(10), 1289-1293 (2006)

Questions

1–5 of 5 Questions  
  1. May I ask if I can use P- phenylenediamine for oxidase test 

    1 answer
    1. We have no information on using this product for oxidase test. Product No. T3134, N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride is commonly used to perform the oxidase test for the colorimetric identification of pathogenic and non-pathogenic bacteria.

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  2. What is the solubility of p-Phenylediamine, Product P6001?

    1 answer
    1. This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. For what applications is p-Phenylediamine, Product P6001, used?

    1 answer
    1. This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.

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  5. What is the working concentration for p-Phenylediamine, Product P6001, when used as an antifade agent?

    1 answer
    1. J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)".

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