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N4762
Newborn Calf Serum
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture
Synonyme(s) :
NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera
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About This Item
Source biologique:
bovine (calf)
Origine:
USA origin
Stérilité:
sterile-filtered
Forme:
liquid
Conditions d'expédition:
dry ice
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
bovine (calf)
Niveau de qualité
Stérilité
sterile-filtered
Forme
liquid
Composition
hemoglobin, ≤20 mg/mL
Origine
USA origin
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤100 EU/mL endotoxin
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Application
NCS is useful in a wide range of cell culture and organ preservation applications. For example, it is used in cornea preservation and cryopreservation of bovine embryos. NCS is used in cell culture to support the maturation of oocytes; to study the function of tubular epithelial cells; and to develop primary cultures of cells such as visceral adipocytes.
Notes préparatoires
Collected from calves that are 10 days old or less.
Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes.
Remarque sur l'analyse
Endotoxin tested
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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Articles
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
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What is the Department of Transportation shipping information for this product?
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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