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MAK246

Sigma-Aldrich

Neutrophil Elastase Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

Synonyme(s) :

Elastase Enzyme Activity Fluorescent Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

hematological disorder; pulmonary disorders

Température de stockage

−20°C

Description générale

Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). Mutation in the gene ELA2 (neutrophil elastase) results in neutropenia, a hereditary disorder characterized by reduced neutrophil count in an individual.[1]

Application


  • Relationship between airway inflammation and airflow limitation in elderly asthmatics.: This study investigates the connection between airway inflammation and airflow limitation in elderly asthmatic patients. It highlights the role of neutrophil elastase in airway inflammation, which was measured using the Neutrophil Elastase Activity Assay Kit (Fluorometric). The findings suggest that targeting neutrophil elastase activity could help in managing airflow limitation in elderly asthmatics, offering potential therapeutic applications for the assay kit (Soma et al., 2020).

Adéquation

Suitable to determine the activity of purified Neutrophil Elastase, detect the activity of Neutrophil Elastase in plasma and/or blood and screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase.

Principe

The Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence. This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase in a variety of samples.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
6M29K03830
8D04K03830
7B26K03830
6G24K03830
5M13K03830

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Neutrophil elastase in cyclic and severe congenital neutropenia.
Horwitz M S, et al.
Blood, 109(5), 1817-1824 (2007)
Sang-Il Han et al.
Bioresource technology, 302, 122791-122791 (2020-01-26)
The effect of co-cultivation of Porphyridium cruentum UTEX 161 with Pseudoalteromonas sp. MEBiC 03485 on P. cruentum growth and its sulfated polysaccharide (EPS) production were examined. The strain MEBiC 03485 had beneficial effects on P. cruentum growth, EPS production, and

Questions

1–2 of 2 Questions  

  1. will this kit work with human and mice sample both?

    1 answer

    1. This kit can be used to determine neutrophil elastase activity from both species. Please review the article citations below where researchers have reported this kit being used with neutrophils isolated from human blood and mice brain.

      Pia Kuschnerow, Antje Munder, Nicole de Buhr, Matthias Mörgelin, Adan Chari Jirmo, Mania Ackermann, Maren von Köckritz-Blickwede, Burkhard Tümmler, Nina Cramer,
      Competitive survival of clonal serial Pseudomonas aeruginosa isolates from cystic fibrosis airways in human neutrophils, iScience, Volume 26, Issue 4, 2023, 106475, ISSN 2589-0042, https://doi.org/10.1016/j.isci.2023.106475.

      Li F, Zhang Y, Li R, Li Y, Ding S, Zhou J, Huang T, Chen C, Lu B, Yu W, Boltze J, Li P, Wan J. Neuronal Serpina3n is an endogenous protector against blood brain barrier damage following cerebral ischemic stroke. J Cereb Blood Flow Metab. 2023 Feb;43(2):241-257. doi: 10.1177/0271678X221113897. Epub 2022 Dec 1. PMID: 36457151; PMCID: PMC9903218.

      Helpful?

  2. Hi I am wondering if you have any references where this assay has been used with Human serum. Please let me know. Thanks.

    1 answer

    1. A detailed search of the scientific literature does not reveal any references to utilizing this kit with human serum. This kit has not been tested directly on serum samples. In theory, it could possibly work as long as the serum sample has active elastase. This may require some research and development to utilize the kit for the intended purpose. Please also keep in mind that the kit is used primarily for elastase from neutrophils which is a type of white blood cell. The serum should be devoid of white blood cells from the collection process.

      Helpful?

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