Accéder au contenu
Merck
Toutes les photos(1)

Documents

LYSISO1

Sigma-Aldrich

Lysosome Isolation Kit

sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CE :
233-140-8
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Utilisation

sufficient for 20 mL (packed cells)
 sufficient for 25 g (tissue)

Technique(s)

centrifugation: suitable
fractionation: suitable

Température de stockage

2-8°C

Description générale

Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.

Application

Lysosome Isolation Kit has been used:
  • in the isolation of cytosol and lysosome
  • to obtain enriched light and heavy lysosomal fractions from cultured cells
  • in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
  • in lysosome purification

The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.

Caractéristiques et avantages

  • Generates functional organelles for metabolism and disease research
  • Includes protease inhibitor to manage degradation
  • Measures integrity with included Neutral Red dye
  • Choose from multiple options to obtain enriched fractions
  • Enriched fractions for intact and functional lysosomes from tissues and cells
  • Suitable for functional studies including protein degradation

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLFDS

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Fang-Shin Nian et al.
Molecular neurobiology, 56(9), 6095-6105 (2019-02-06)
Mutations in RAB18, a member of small G protein, cause Warburg micro syndrome (WARBM), whose clinical features include vision impairment, postnatal microcephaly, and lower limb spasticity. Previously, our Rab18-/- mice exhibited hind limb weakness and spasticity as well as signs
Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections
Xu B, et al.
Molecular Immunology, 87(1), 122-131 (2017)
Xiaowei Ma et al.
ACS nano, 5(11), 8629-8639 (2011-10-07)
Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. Autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. Previous studies have shown that nanoparticles from various sources
Emil Rudolf et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(1), 1-16 (2017-08-30)
Zinc (Zn) is an important microelement required by skin cells for a variety of biological processes. The role of Zn in melanocyte proliferation and homeostasis has to date not been investigated. Human dermal melanocytes were isolated from patients and their
Facilitated mitochondrial import of anti-viral and anti-cancer nucleoside drugs by human equilibrative nucleoside transporter-3 (hENT3)
Govindarajan R, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology (2009)
Afficher tous les articles scientifiques apparentés

Articles

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique