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D9154

Sigma-Aldrich

o-Dianisidine dihydrochloride

tablet

Synonyme(s) :

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Formule empirique (notation de Hill):
C14H16N2O2 · 2HCl
Numéro CAS:
20325-40-0
Poids moléculaire :
317.21
Numéro Beilstein :
3917996
Numéro CE :
243-737-5
Numéro MDL:
MFCD00012488
Code UNSPSC :
12352204
ID de substance PubChem :
24894263
Nomenclature NACRES :
NA.83

product name

o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet

Forme

tablet

Solubilité

water: 1 tablet/10 mL, clear, colorless

Température de stockage

2-8°C

Chaîne SMILES 

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

Clé InChI

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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Spécificité

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Avertissement

Probably carcinogenic.

Reconstitution

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H302,H314,H350

Classification des risques

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

M Kaszuba et al.
Biochimica et biophysica acta, 1419(2), 221-228 (1999-07-17)
Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated
Frédéric Frottin et al.
Molecular & cellular proteomics : MCP, 5(12), 2336-2349 (2006-09-12)
Methionine aminopeptidase (MAP) is a ubiquitous, essential enzyme involved in protein N-terminal methionine excision. According to the generally accepted cleavage rules for MAP, this enzyme cleaves all proteins with small side chains on the residue in the second position (P1')
Cathrine Nordgaard et al.
International journal of molecular sciences, 22(17) (2021-09-11)
p38 and c-Jun N-terninal kinase (JNK) are activated in response to acute stress and inflammatory signals. Through modification of a plethora of substrates, these kinases profoundly re-shape cellular physiology for the optimal response to a harmful environment and/or an inflammatory
Rémy Ricoux et al.
Bioconjugate chemistry, 19(4), 899-910 (2008-03-08)
To develop artificial hemoproteins that could lead to new selective oxidation biocatalysts, a strategy based on the insertion of various iron-porphyrin cofactors into Xylanase A (Xln10A) was chosen. This protein has a globally positive charge and a wide enough active
Yanfen Peng et al.
Analytical chemistry, 83(3), 820-827 (2011-01-07)
A highly sensitive microRNA (miRNA) biosensor that employs ruthenium oxide nanoparticle (RuO(2) NP)-initiated polymerization of 3,3'-dimethoxybenzidine (DB) and miRNA-templated deposition of an insulating poly(3,3'-dimethoxybenzidine) (PDB) film is described in this work. The biosensor was made of a mixed monolayer of
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