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F5803

Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Synonyme(s) :

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Formule empirique (notation de Hill):
C14H16N2O2 · 2HCl
Numéro CAS:
20325-40-0
Poids moléculaire :
317.21
Numéro Beilstein :
3917996
Numéro CE :
243-737-5
Numéro MDL:
MFCD00012488
Code UNSPSC :
12171500
ID de substance PubChem :
24894894
Nomenclature NACRES :
NA.47

Forme

crystalline

Plage de pH

6.95-7.05

Pf

268 °C

Solubilité

water: 2.5 mg/mL, colorless

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Chaîne SMILES 

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

Clé InChI

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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Description générale

A preweighed vial containing 50 mg o-Dianisidine.

Spécificité

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
  • Myeloperoxidase activity assay.

Avertissement

Probably carcinogenic.

Enzyme

Réf. du produit
Description
Tarif

Étalon

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H302,H314,H350

Classification des risques

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Wijitar Dungchai et al.
Analytica chimica acta, 674(2), 227-233 (2010-08-04)
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for
Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
K H Schosinsky et al.
Clinical chemistry, 20(12), 1556-1563 (1974-12-01)
Risheng Ye et al.
Molecular metabolism, 5(7), 437-448 (2016-07-14)
Evidence hints at the ability of β-cells to emerge from non-β-cells upon genetic or pharmacological interventions. However, their quantitative contributions to the process of autonomous β-cell regeneration without genetic or pharmacological manipulations remain to be determined. Using PANIC-ATTAC mice, a
G B Penner et al.
Journal of dairy science, 92(4), 1725-1733 (2009-03-25)
This study was conducted to determine the effects of feeding Fermenten (Church and Dwight Co., Princeton, NJ) with or without dietary sucrose on ruminal fermentation, apparent total-tract nutrient digestibility, and nutrient utilization. Eight ruminally cannulated Holstein cows (163 +/- 55
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted
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Protocoles

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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