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C4129
α-Chymotrypsin from bovine pancreas
Type II, lyophilized powder, ≥40 units/mg protein
Synonyme(s) :
α-chymotrypsin A and B, alpha-chymotrypsin
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About This Item
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Produits recommandés
Type
Type II
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
≥40 units/mg protein
Poids mol.
25 kDa
Composition
protein, ≥85%
Couleur
white to off-white
Numéro d'accès UniProt
Application(s)
diagnostic assay manufacturing
Température de stockage
−20°C
Informations sur le gène
cow ... CTRB1(618826)
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Catégories apparentées
Application
The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).[1]
Actions biochimiques/physiologiques
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Notes préparatoires
Produced from 3× crystallized chymotrypsinogen
Remarque sur l'analyse
Protein determined by E1%/280
Autres remarques
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
Application
Réf. du produit
Description
Tarif
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Articles
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Protocoles
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
Chromatograms
application for HPLC-
What is the Department of Transportation shipping information for this product?
1 answer-
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How can solutions of alpha chymotrypsin (Product No. C4129) be stored?
1 answer-
Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
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What is the pH optimum for the enzyme, Product No. C4129?
1 answer-
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
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How do you recommend preparing a solution of Product No. C4129?
1 answer-
It is recommended to reconstitute Product No. C4129 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as a stabilizer, and possibly an activator, of the enzyme.
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What is the molecular weight of alpha-chymotrypsin from bovine pancreas, product C4129?
1 answer-
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.
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