A2356
Phosphatase, Alkaline from bovine intestinal mucosa
≥5,500 DEA units/mg protein
Synonyme(s) :
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
42010105
Nomenclature NACRES :
NA.54
Produits recommandés
Forme
(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
Niveau de qualité
Activité spécifique
≥5,500 DEA units/mg protein
Poids mol.
dimer ~160 kDa
Concentration
≥10 mg/mL
Température de stockage
2-8°C
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Application
Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.
Actions biochimiques/physiologiques
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.
Propriétés physiques
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.
Définition de l'unité
1 DEA-Einheit hydrolysiert 1 μmol 4-Nitrophenyl-Phosphat pro Minute bei pH 9.8 und 37 °C.
Forme physique
Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6
Remarque sur l'analyse
Package sizes are based on DEA units
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
Certificats d'analyse (COA)
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Protocoles
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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