P4069

Phosphatase, Alkaline from Escherichia coli

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

• Synonyme(s) : Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Numéro CAS: 9001-78-9
• Numéro de classification (Commission des enzymes): 3.1.3.1 (BRENDA, IUBMB)
• Numéro CE : 232-631-4
• Numéro MDL: MFCD00131849
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Escherichia coli
• Niveau de qualité: 200
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 20-50 units/mg protein (in glycine buffer)
• Activité étrangère: DNase, RNase, none detected
• Température de stockage: −20°C

Description

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Phosphatase, Alkaline from Escherichia coli has been used:

• in fluorometric assay to determine its enzyme activity
• in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
• in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Actions biochimiques/physiologiques

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Attention

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Forme physique

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl₂, 0.5 mM ZnCl₂, 50% (w/v) glycerol

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)