The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
11191
Histopaque®-1119
Histopaque®-1119
sterile-filtered, density: 1.119
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Stérilité
sterile-filtered
Niveau de qualité
Forme
liquid
Durée de conservation
Expiry date on the label.
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Densité
1.119
Application(s)
hematology
histology
Température de stockage
2-8°C
Description générale
Le produit Histopaque®-1119 est un milieu stérile prêt à l'emploi dépourvu d'endotoxines (tests à l'appui). C'est une solution de polysaccharose et de diatrizoate de sodium ajustée à une densité de 1,119 g/ml et à un pH de 8,8 à 9,0.
Application
La solution Histopaque®-1119 peut être associée au produit Histopaque-1077 pour récupérer rapidement les cellules mononucléaires et les granulocytes viables présents dans de petits volumes de sang total ou de moelle osseuse.
Caractéristiques et avantages
Histopaque®-1119 présente de nombreux avantages, notamment :
- Recouvrement optimal des cellules viables
- Permet de séparer sélectivement les différents types de cellules sanguines
- Évite la déformation des cellules
- Préserve la viabilité cellulaire
- Interférences minimales avec les cellules exogènes
- Performances reproductibles d'un lot à l'autre
- Produit stérilisé par filtration et trouvé négatif pour la recherche d'endotoxines
- Produit stable entre 2 °C et 8 °C à l'abri de la lumière pendant au moins 2 ans.
Principe
Il s'agit de former un double gradient lors du dépôt d'une couche d'Histopaque®-1077 sur une couche d'Histopaque®-1119, toutes deux en volumes égaux. Le sang total anticoagulé est étalé délicatement sur la couche supérieure d'Histopaque®-1077. À la centrifugation, les érythrocytes sont agrégés par le polysaccharose et sédimentent donc rapidement. Les granulocytes se retrouvent au niveau de l'interface inférieure Histopaque®-1077/Histopaque ®1119 tandis que les lymphocytes et les autres cellules mononucléaires se trouvent au niveau de l'interface supérieure plasma/Histopaque®-1077. La plupart des plaquettes exogènes présentes au niveau de l'interface supérieure sont éliminées par centrifugation à basse vitesse lors des étapes de lavage. La contamination érythrocytaire est négligeable.
Informations légales
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Protocoles
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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Is there a product similar to Mono-Poly resolving media?
1 answer-
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
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The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
1 answer-
Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 answer-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
1 answer-
This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05Helpful?
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The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
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Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 answer-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
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No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
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When using Product 11191, Histopaque®-1119, what is the expected percent recovery for the band containing neutrophils.
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No percent recovery data has been generated. Due to the importance of technique with the Histopaque 1119-1, percent recovery varies with the skill of the operator.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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