B2261

bisBenzimide H 33342 trihydrochloride

≥98% purity (HPLC and TLC), powder

• Synonyme(s) : 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
• Formule empirique (notation de Hill) : C₂₇H₂₈N₆O · 3HCl · xH₂O
• Numéro CAS: 875756-97-1
• Poids moléculaire : 561.93 (anhydrous basis)
• Beilstein: 1234011
• Numéro MDL: MFCD00012678
• Code UNSPSC : 12171500
• ID de substance PubChem : 24891639
• Nomenclature NACRES : NA.47

Propriétés

• Nom du produit: bisBenzimide H 33342 trihydrochloride, ≥98% (HPLC and TLC)
• Niveau de qualité: 300
• Essai: ≥98% (HPLC and TLC)
• Forme: powder
• Technique(s): titration: suitable
• Couleur: yellow
• pH: 1.7 (20 °C)
• Solubilité: H₂O: 20 mg/mL; phosphate buffer: precipitates
• Adéquation: suitable for fluorescence
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
• InChI: 1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
• Clé InChI: FYEVKHPLBHLWHK-UHFFFAOYSA-N

Description

Application

Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in living cells. It has been used to detect BrdU incorporation into cells and in studying the initial stages apoptosis and cellcycle distribution.^, Chromosomes that are dividing or replicating will not stain with this dye.

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Actions biochimiques/physiologiques

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Propriétés physiques

Fluorescent properties of bisBenzimide H 33342:
Free dye: Excitation maximum = 340 nm, Emission maximum = 510 nm (5 mM HEPES, 10 mM NaCl, pH 7.0) DNA complex: Excitation maximum = 355 nm, Emission maximum = 465 nm (5 mM HEPES, 10 mM NaCl, pH 7.0)

Notes préparatoires

This product is soluble in water (50 mg/ml), yielding a clear solution. The pH of a 2% solution is 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.
Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable