Genomic DNA purified by phenol chloroform extraction is sufficiently clean for use with this kit.
DNAQF
DNA Quantitation Kit, Fluorescence Assay
Quantitation of DNA using bisBenzimide
Synonyme(s) :
dsDNA quantification
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41105500
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Niveau de qualité
Utilisation
sufficient for 750 assays (2 ml each)
Technique(s)
nucleic acid detection: suitable
Température de stockage
−20°C
Description générale
The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins.[1][2][3] Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.
Application
DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
- in neonatal rat ventricular myocytes (NRVMs)[4]
- in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount[5]
- in mouse embryonic fibroblast cell line (NIH3T3 cells)[6]
Caractéristiques et avantages
- Specific for the quantitation of nanogram amounts of DNA
- Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins
Composants de kit seuls
Réf. du produit
Description
- Detailed technical bulletin 1 ea
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
J M Teare et al.
BioTechniques, 22(6), 1170-1174 (1997-06-01)
Molecular biology is now a routine tool in almost all biological research fields. With the exponential growth in the number of molecular biological techniques, there is a recognizable need for sensitive, accurate and precise quantitation of nucleic acids. We present
The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware.
Weiszenstein M
PLoS ONE, 11(3), e0152382-e0152382 (2016)
Claire G Jeong et al.
Journal of biomedical materials research. Part A, 100(8), 2088-2096 (2012-05-23)
As articular cartilage is avascular, and mature chondrocytes do not proliferate, cartilage lesions have a limited capacity for regeneration after severe damage. The treatment of such damage has been challenging due to the limited availability of autologous healthy cartilage and
Steve Elder et al.
Journal of biomedical materials research. Part A, 106(8), 2251-2260 (2018-03-27)
Given the limited availability of fresh osteochondral allografts and uncertainty regarding performance of decellularized allografts, this study was undertaken as part of an effort to develop an osteochondral xenograft for articular cartilage repair. The purpose was to evaluate a simple
Kausik Kapat et al.
ACS applied materials & interfaces, 9(45), 39235-39248 (2017-10-24)
Cementless fixation for orthopedic implants aims to obviate challenges associated with bone cement, providing long-term stability of bone prostheses after implantation. The application of porous titanium and its alloy-based implants is emerging for load-bearing applications due to their high specific
-
What quality of genomic DNA is required for use in Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?
1 answer-
Helpful?
-
-
How can I ensure accurate initial quantification of the DNA standard by spectrophotometric measurement at A260 when using Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?
1 answer-
The DNA standard (Product No. D4810) should be incubated at 50 °C for 15-30 minutes before determining the concentration spectrophotometrically. This will ensure complete dissolution of the genomic DNA.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
I see that one of the componets of Product DNAQF, DNA Quantitation Kit, Fluorescence Assay, is a DNA standard. How is the DNA standard prepared? Can it be purchased separately?
1 answer-
The DNA standard (Product No. D4810) used in the kit is a sheared calf thymus DNA. It is the only one tested with the kit and it is only available in the kit. We do not determine the molecular weight.
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
