DAPI is suitable for staining bacterial chromosomes, including those of E. coli.
D9542
DAPI
DAPI
for nucleic acid staining
Synonyme(s) :
4′,6-Diamidino-2-phénylindole dihydrochloride, 2-(4-Amidinophényl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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About This Item
Formule empirique (notation de Hill) :
C16H15N5 · 2HCl
Numéro CAS:
Poids moléculaire :
350.25
Beilstein:
4894417
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352200
ID de substance PubChem :
Nomenclature NACRES :
NA.52
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Qualité
for molecular biology
Essai
≥98% (HPLC)
Forme
powder
Technique(s)
transfection: suitable
Solubilité
H2O: 20 mg/mL
PBS: insoluble
ε (coefficient d'extinction)
30 at 263 nm in H2O at 1 mM
Fluorescence
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
Adéquation
suitable for fluorescence
Température de stockage
2-8°C
Chaîne SMILES
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
Clé InChI
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Catégories apparentées
Description générale
Le DAPI (dichlorhydrate de 4′,6-Diamidino-2-phénylindole) est un colorant fluorescent capable de pénétrer dans les cellules et de se lier à l′ADN.
la chaleur ou la sonication peuvent être nécessaires. Les solutions stockées à l'abri de la lumière à température ambiante ou à 4 °C devraient être stables pendant 2 à 3 semaines.
Application
Adapté aux applications suivantes :
Le DAPI a été utilisé :
- Coloration de l′ADN dans des gels d′agarose
- Analyse des modifications de l′ADN lors de l′apoptose
- Détection des mycoplasmes
- Photofootprinting de l′ADN
- Coloration par immunofluorescence des cellules
Le DAPI a été utilisé :
- pour la surveillance rapide de la contamination microbiologique[1]
- dans la technique de coloration des bandes de chromosomes[2]
- pour la détection des cellules apoptotiques[3]
- en microscopie à fluorescence, pour suivre le mouvement de la protéine DisA (DNA integrity scanning protein) sur l′ADN de Bacillus subtilis[4]
- pour colorer les grains de pollen matures (0,5 mg/ml)[5]
Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose.
Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose. Il peut être utilisé dans le photofootprinting de l′ADN, pour détecter les sondes hybridées dans les applications sur membranes en permettant la visualisation spécifique des complexes double-brins, et pour évaluer les changements de l′ADN et de son contenu lors de l′apoptose par cytométrie en flux. La coloration par DAPI est également une méthode sensible et spécifique de détection des mycoplasmes.
Actions biochimiques/physiologiques
Sonde fluorescente pour l′ADN qui se lie dans le petit sillon, capable de pénétrer dans les cellules. Se lie au petit sillon d′ADN double brin (préférentiellement à l′ADN riche en AT), formant un complexe stable dont l′intensité de fluorescence est environ 20 fois plus élevée que celle du DAPI non lié.
Attention
Protéger de la lumière.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and
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Protocoles
ICC Cell Capture Imaging Reagent simplifies imaging, ideal for rare cell and low cell count samples.
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Can this product be used to stain bacteria (E.coli) chromosome?
1 answer-
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What is the working dilution of DAPI that should be used to stain the nuclei of human cells?
1 answer-
Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdfPlease see the link below to review a sample protocol from Cold Spring Harbor:
https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.longHelpful?
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what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?
1 answer-
This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfHelpful?
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Can the stain concentration be used for staining fish scales and any animal cells for ICC?
1 answer-
Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.
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What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?
1 answer-
Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.
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What is the suggested concentration for product D9542-1MG?
1 answer-
The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.
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肝細胞を染める染色プロトコルを教えてください。
1 answer-
Please see the link below to review the product datasheet, which includes basic protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfA protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/Helpful?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?
1 answer-
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation. At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?
1 answer-
This product is soluble in water at 20 mg/mL, but may require heat. DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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