Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

11685619910

Roche

Fluorescein RNA Labeling Mix

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

Synonyme(s) :

RNA labeling

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41105500

Forme

solution

Niveau de qualité

100

Utilisation

sufficient for 20 reactions

Conditionnement

pkg of 40 μL

Fabricant/nom de marque

Roche

Technique(s)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

Température de stockage

−20°C

Catégories apparentées

Description générale

Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).

Spécificité

Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).

Application

Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
  • in situ hybridization to tissues
  • Southern blots
  • northern blots
  • plaque or colony lifts
  • RNase protection experiments

Qualité

The mix is function tested in combination with the DIG RNA Labeling Kit.

Principe

Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.

Notes préparatoires

10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
59973820
41027920
32874620
24158720
70036320

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 5

1 of 5

T7 RNA Polymerase from Escherichia coli BL 21/pAR 1219

Roche

RPOLT7-RO

T7 RNA Polymerase

Digoxigenin-11-UTP

Roche

DIGUTP-RO

Digoxigenin-11-UTP

T3 RNA Polymerase from Escherichia coli HB101

Roche

RPOLT3-RO

T3 RNA Polymerase

ARN polymérase SP6 from Escherichia coli BL 21/pSR3

Roche

RPOLSP6-RO

ARN polymérase SP6

INT/BCIP Stock Solution solution, pkg of 3 mL

Roche

11681460001

INT/BCIP Stock Solution

Zhimin Jiang et al.
Nature microbiology, 6(7), 932-945 (2021-05-15)
The retinoic acid-inducible gene I (RIG-I) receptor senses cytoplasmic viral RNA and activates type I interferons (IFN-I) and downstream antiviral immune responses. How RIG-I binds to viral RNA and how its activation is regulated remains unclear. Here, using IFI16 knockout
Yasufumi Hayano et al.
Science advances, 7(29) (2021-07-16)
The most prominent structural hallmark of the mammalian neocortical circuitry is the layer-based organization of specific cell types and synaptic inputs. Accordingly, cortical inhibitory interneurons (INs), which shape local network activity, exhibit subtype-specific laminar specificity of synaptic outputs. However, the
Lucia Caceres et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 248(12), 1243-1256 (2019-10-01)
Familial exudative vitreoretinopathy (FEVR) is a rare congenital disorder characterized by a lack of blood vessel growth to the periphery of the retina with secondary fibrovascular proliferation at the vascular-avascular junction. These structurally abnormal vessels cause leakage and hemorrhage, while
Anke J Roelofs et al.
Annals of the rheumatic diseases, 79(12), 1625-1634 (2020-09-24)
Osteophytes are highly prevalent in osteoarthritis (OA) and are associated with pain and functional disability. These pathological outgrowths of cartilage and bone typically form at the junction of articular cartilage, periosteum and synovium. The aim of this study was to
Paul A Dawson et al.
Biology of reproduction, 87(2), 43-43 (2012-06-08)
Sulfate is important for mammalian growth and development. During pregnancy, maternal circulating sulfate levels increase by 2-fold, enhancing sulfate availability to the fetus. We used quantitative real-time PCR to determine sulfate transporter mRNA levels during mouse gestation in three tissues:
Afficher tous les articles scientifiques apparentés

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique