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DIGUTP-RO

Roche

Digoxigenin-11-UTP

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.21

Pureté

≥85% (HPLC)

Niveau de qualité

100

Forme

solution

Poids mol.

(Mr = 1106.7 (DIG-11-UTP-Li4))

Conditionnement

pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])

Fabricant/nom de marque

Roche

Température de stockage

−20°C

Description générale

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:
  • in situ hybridization
  • in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
  • fluorescence in situ hybridization (FISH)

Actions biochimiques/physiologiques

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Qualité

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C43H61N4O22P3Li4

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H302

Classification des risques

Acute Tox. 4 Oral

Code de la classe de stockage

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution
<BIG>Chen X, et al.</BIG>
Biotechniques, 56 (2014)
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)
A George et al.
Connective tissue research, 33(1-3), 67-72 (1995-01-01)
Acidic phosphorylated proteins are prominent constituents of the extracellular matrix of bone and dentin. It has been postulated that they may have important structural and regulatory roles in the process of tissue mineralization. Studies of a cDNA library, prepared from
Isolation and functional characterization of two dioxygenases putatively involved in bixin biosynthesis in annatto (Bixa orellana L.)
<BIG>Carballo U, et al.</BIG>
PeerJ, 7 (2019)
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