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DIGUTP-RO

Roche

Digoxigenin-11-UTP

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A propos de cet article

NACRES:
NA.21
UNSPSC Code:
41105500
Assay:
≥85% (HPLC)
Form:
solution
Storage temp.:
−20°C
Service technique
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assay

≥85% (HPLC)

form

solution

mol wt

(Mr = 1106.7 (DIG-11-UTP-Li4))

packaging

pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:
  • in situ hybridization
  • in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
  • fluorescence in situ hybridization (FISH)

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Analysis Note

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C43H61N4O22P3Li4

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

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Certificats d'analyse (COA)

Lot/Batch Number
86106221
86106220
63347022
63347021
60007521

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Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)
A George et al.
Connective tissue research, 33(1-3), 67-72 (1995-01-01)
Acidic phosphorylated proteins are prominent constituents of the extracellular matrix of bone and dentin. It has been postulated that they may have important structural and regulatory roles in the process of tissue mineralization. Studies of a cDNA library, prepared from
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
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Numéro d'article de commerce international

RéférenceGTIN
335924791004061826742723
1120925691004061837902062