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Key Documents

11051482001

Roche

dTTP

Lithium salt

Synonyme(s) :

Thymidine 5′-triphosphate sodium salt, 2′-deoxy-thymidine-5′-triphosphate, deoxy-thymidine-5′-triphosphate, 2′-, TTP, dTTP

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About This Item

Formule empirique (notation de Hill):
C10H16N2O14P3Na
Numéro CAS:
Poids moléculaire :
504.15
Numéro MDL:
Code UNSPSC :
41116100
ID de substance PubChem :

Niveau de qualité

Pureté

≥98%

Forme

solution

Utilisation

sufficient for 6,250 standard reactions (PCR)

Conditionnement

pkg of 250 μL (25 μmol)

Fabricant/nom de marque

Roche

Concentration

100 mM

Technique(s)

DNA amplification: suitable
DNA sequencing: suitable

Température de stockage

−20°C

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

Clé InChI

KAELIXTVSANDSE-UHFFFAOYSA-N

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Description générale

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

Application

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.

Qualité

Typical analysis:
  • ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Définition de l'unité

1 μmol dTTP = 9.5 A260units

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Wieland Meyer et al.
Emerging infectious diseases, 9(2), 189-195 (2003-02-27)
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase
Luciana Trilles et al.
Memorias do Instituto Oswaldo Cruz, 103(5), 455-462 (2008-09-18)
The molecular types of 443 Brazilian isolates of Cryptococcus neoformans and Cryptococcus gattii were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture
A Pillay et al.
Journal of clinical microbiology, 42(8), 3853-3856 (2004-08-07)
An immunochromatographic strip test, Xenostrip-Tv, was compared to wet mount and PCR for the diagnosis of Trichomonas vaginalis infection in women. Of 428 specimens tested, 54 (12.6%) were positive by an "expanded gold standard," defined as either a positive wet

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