MR-101
EmbryoMax® Advanced KSOM Embryo Medium
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
Synonyme(s) :
K+ Simplex Optimised Medium (KSOM), KSOM Mouse Embryo Media
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About This Item
Code UNSPSC :
12352207
eCl@ss :
32160801
Nomenclature NACRES :
NA.71
Produits recommandés
Niveau de qualité
Forme
liquid
Fabricant/nom de marque
Specialty Media
EmbryoMax®
Technique(s)
cell culture | embryo: suitable
cell culture | stem cell: suitable
Entrée
sample type: mouse embryo(s)
Description générale
EmbryoMax Advanced KSOM Medium is a new, uniquely buffered modified version of KSOM that can be used as a single medium to harvest and culture preimplantation mouse embryos. The medium will maintain pH out on the bench top and also grow embryos in vitro, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture. The Advanced KSOM medium has been shown to produce a higher frequency of blastocyst formation in multiple mouse strains after 72 hours in culture when compared to standard KSOM medium.
Traditionally, retrieving and then growing mouse embryos in vitro is usually a 2-step system. Embryos are commonly removed from the oviducts in a HEPES buffered medium (e.g. M2, FHM), which maintain their pH in air. The embryos are then transferred into a second medium which is bicarbonate buffered (e.g. M16, CZB, KSOM) and placed in a CO2 incubator in order to maintain the pH of the medium. Furthermore, the bicarbonate buffered medium needs to be pre-equilibrated in the incubator for several hours prior to receiving embryos to achieve optimal growth. The EmbryoMax Advanced KSOM Medium enables both embryo handling in atmospheric conditions and culturing in a CO2 incubator with a single medium. No medium pre-equilibration step is necessary for embryo harvest, but pre-equilibration is recommended for culture.
Traditionally, retrieving and then growing mouse embryos in vitro is usually a 2-step system. Embryos are commonly removed from the oviducts in a HEPES buffered medium (e.g. M2, FHM), which maintain their pH in air. The embryos are then transferred into a second medium which is bicarbonate buffered (e.g. M16, CZB, KSOM) and placed in a CO2 incubator in order to maintain the pH of the medium. Furthermore, the bicarbonate buffered medium needs to be pre-equilibrated in the incubator for several hours prior to receiving embryos to achieve optimal growth. The EmbryoMax Advanced KSOM Medium enables both embryo handling in atmospheric conditions and culturing in a CO2 incubator with a single medium. No medium pre-equilibration step is necessary for embryo harvest, but pre-equilibration is recommended for culture.
Application
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
Research Category
Stem Cell Research
Stem Cell Research
Stockage et stabilité
Can be stored at -20°C up to the expiration date on the bottle.
Once thawed it should be used within 2 weeks.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.
Once thawed it should be used within 2 weeks.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.
Informations légales
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
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