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Key Documents

07-551

Sigma-Aldrich

Anti-G9a Antibody

Upstate®, from rabbit

Synonyme(s) :

KMT1C

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human

Conditionnement

antibody small pack of 25 μg

Fabricant/nom de marque

Upstate®

Technique(s)

western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EHMT2(10919)

Description générale

Protein G9a (also known as Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2 and HLA-B-associated transcript 8)

Spécificité

Recognizes human G9a protein.

Immunogène

Peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-G9a Antibody (Rabbit Polyclonal Antibody) validated in WB to detect G9a also known as KMT1C.

Qualité

routinely evaluated by immunoblot HeLa nuclear extract cell lysate

Description de la cible

140 kDa

Forme physique

Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified

Stockage et stabilité

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Control
Positive Control Included: HeLa Nuclear Extract (12-309).

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex.
Kuppuswamy, M; Vijayalingam, S; Zhao, LJ; Zhou, Y; Subramanian, T; Ryerse, J; Chinnadurai, G
Molecular and cellular biology null
Changes in C-terminal binding protein 2 (CtBP2) corepressor complex induced by E1A and modulation of E1A transcriptional activity by CtBP2.
Zhao, LJ; Subramanian, T; Chinnadurai, G
The Journal of Biological Chemistry null
Gfi1 coordinates epigenetic repression of p21Cip/WAF1 by recruitment of histone lysine methyltransferase G9a and histone deacetylase 1.
Duan, Z; Zarebski, A; Montoya-Durango, D; Grimes, HL; Horwitz, M
Molecular and cellular biology null
Mihai G Mehedint et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(1), 184-195 (2009-09-16)
Maternal choline availability is essential for fetal neurogenesis. Choline deprivation (CD) causes hypomethylation of specific CpG islands in genes controlling cell cycling in fetal hippocampus. We now report that, in C57BL/6 mice, CD during gestational days 12-17 also altered methylation
Seth Frietze et al.
PloS one, 5(12), e15082-e15082 (2010-12-21)
Only a small percentage of human transcription factors (e.g. those associated with a specific differentiation program) are expressed in a given cell type. Thus, cell fate is mainly determined by cell type-specific silencing of transcription factors that drive different cellular

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