05-485

Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

clone 9E12, Upstate^®, from mouse

• Synonyme(s) : Acute-phase response factor, DNA-binding protein APRF, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase response factor)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 9E12, monoclonal
• Species reactivity: canine, human, mouse
• Application: ELISA, IP, WB
• Citations: 26

Propriétés

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9E12, monoclonal
• species reactivity: canine, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_139276
• UniProt accession no.: P40763
• shipped in: dry ice
• target post-translational modification: phosphorylation (pTyr705)
• Gene Information: human ... STAT3(6774)

Description

General description

92 kDa

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

Immunogen

KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3

Application

Domaine de recherche
Épigénétique et fonction nucléaire

Immunoprécipitation : µ

µ Test was conducted on a previous lot.

Sous-domaine de recherche
Facteurs de transcription

Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF. This Stat3 Antibody has been validated in Human, Mouse and Canine samples.

Biochem/physiol Actions

This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.

Physical form

ќ µ

Adsorption thiophile et chromatographie par exclusion de taille

Format : Produit purifié

Preparation Note

Stable à -20 °C pendant 1 an à compter de la date de réception.

Recommandations relatives à la manipulation du produit :
dès réception, et avant retrait du bouchon, centrifuger le flacon et mélanger délicatement la solution. Répartir en aliquotes dans des microtubes à centrifuger et conserver ces derniers à -20 °C. Éviter les congélations/décongélations répétées, qui peuvent détériorer les IgG et nuire aux performances du produit. Remarque : Les variations de température à l'intérieur des congélateurs en-dessous de -20 °C peuvent provoquer la congélation des solutions à base de glycérol durant le stockage.

Analysis Note

µ

Other Notes

Concentration : pour connaître la concentration spécifique du lot, voir le certificat d'analyse.

Remplace le(s) produit(s) suivant(s) : 04-1059

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• pictograms: GHS06
• signalword: Danger
• hcodes: H302 + H332,H311,H412
• pcodes: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
• Classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1