HPLC Analysis of Proteins on BIOshell A400 Protein C18, Hydrophobicity Comparision
Matériaux
Colonne analytique
Réf. du produit
Description
Tarif
Étalon
Réf. du produit
Description
Tarif
Superoxide Dismutase from bovine erythrocytes
BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell cultureGlucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)CONDITIONS
column
BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)
mobile phase
[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
gradient
10 to 100% B in 18 min
flow rate
0.3 mL/min
pressure
2800 psi total pressure (193 bar)
column temp.
30 °C
detector
UV, 215 nm
injection
5 μL
sample
superoxide dismutase, 0.13; a-lactalbumin, 0.13; serum albumin bovine, 0.13; ß-lactoglobulin A, 0.06; catalase, 0.13; (carbonic anhydrase, 0.13; ß-amylase, 0.13; glucose-6 phosphate dehydrogenase, 0.13 g/L in mobile phase A)
Description
Remarque sur l'analyse
Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
Informations légales
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