2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).
Y2377
2x YT medium
Powder microbial growth medium
Synonyme(s) :
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
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250 G
77,10 $
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Changer de vue
250 G
77,10 $
About This Item
Code UNSPSC :
41106225
Nomenclature NACRES :
NA.85
Produits recommandés
Qualité
Molecular Biology
for molecular biology
Stérilité
non-sterile
Forme
powder
Technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.1% solution)
Application(s)
food and beverages
microbiology
Température de stockage
room temp
Adéquation
nonselective for Escherichia coli
nonselective for coliforms
Description générale
2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for culturing E. coli BL21 for cloning [1], E. coli SS320 cells for scaffold synthesis [2], and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens[3] and E. coli DH10B.[4]
Caractéristiques et avantages
2xYT powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Composants
16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl
10 g/L Yeast Extract
5 g/L NaCl
Notes préparatoires
1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
Reconstitution
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Protocoles
General protocols for growth of competent cells in microbial medium.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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