Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Documents

Y2021

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

Synonyme(s) :

Yeast Synthetic Drop-out Media Supplements

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD01095679
Code UNSPSC :
41106212
Nomenclature NACRES :
NA.85

Niveau de qualité

200

Forme

powder

Technique(s)

transformation: suitable

Application(s)

microbiology

Température de stockage

room temp

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Notes préparatoires

Dissolve 1.39 g of supplement per liter of medium.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

G7021

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

Gélose, for molecular biology

Y1501

Yeast Synthetic Drop-out Medium Supplements, without uracil

Y1376

Yeast Synthetic Drop-out Medium Supplements, without leucine

Y0626

Base azotée pour levures sans acides aminés, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1876

Yeast Synthetic Drop-out Medium Supplements, without tryptophan

Y1771

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan

Y1896

Yeast Synthetic Drop-out Medium Supplements, without lysine

Y2146

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H318

Conseils de prudence

P280 - P305 + P351 + P338

Classification des risques

Eye Dam. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Elham Alzyoud et al.
Frontiers in cell and developmental biology, 9, 727264-727264 (2021-10-19)
Microtubule nucleation in eukaryotes is primarily promoted by γ-tubulin and the evolutionary conserved protein complex, γ-Tubulin Ring Complex (γ-TuRC). γ-TuRC is part of the centrosome and basal body, which are the best-known microtubule-organizing centers. Centrosomes undergo intensive and dynamic changes
Corinne A Tovey et al.
Current biology : CB, 28(14), 2314-2323 (2018-07-10)
Microtubules are essential for various cell processes [1] and are nucleated by multi-protein γ-tubulin ring complexes (γ-TuRCs) at various microtubule organizing centers (MTOCs), including centrosomes [2-6]. Recruitment of γ-TuRCs to different MTOCs at different times influences microtubule array formation, but

Protocoles

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique