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Sigma-Aldrich

Extract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Synonyme(s) :

Plant direct PCR kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

Caractéristiques

dNTPs included
hotstart

Technique(s)

PCR: suitable

Couleur

colorless

Application(s)

agriculture

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Description générale

The Extract-N-Amp Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

Application

Extract-N-Amp Plant PCR Kit has been used for genomic DNA isolation from plants and the fungal mycelium. It has also been used in polymerase chain reaction (PCR).
Extract-N-Amp Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Caractéristiques et avantages

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Composants

Extract-N-Amp Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

Autres remarques

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

Informations légales

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas
Obase K, et al.
Mycoscience (2021)
Shane Wright et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(20), 7718-7722 (2006-05-05)
Using an appropriately designed and replicated study of a latitudinal influence on rates of evolution, we test the prediction by K. Rohde [(1992) Oikos 65, 514-527] that the tempo of molecular evolution in the tropics is greater than at higher
Ante Karoglan et al.
Acta dermato-venereologica, 99(13), 1253-1257 (2019-10-02)
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
S A Weller et al.
Journal of microbiological methods, 70(2), 379-383 (2007-06-26)
Real-time (TaqMan) PCR assays were developed to detect the strawberry angular leaf spot pathogen Xanthomonas fragariae (Xf) and the strawberry bacterial blight pathogen Xanthomonas arboricola pv. fragariae (Xaf). The Xf PCR (Xf gyrB) was designed within regions of the gyraseB

Articles

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Protocoles

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Contenu apparenté

Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique