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TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

Synonyme(s) :

mitochondrial activity assay

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84

Utilisation

 kit sufficient for 1,000 tests

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

λmax

570 nm

Application(s)

cell analysis
detection

Méthode de détection

colorimetric

Température de stockage

2-8°C

Catégories apparentées

Description générale

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Actions biochimiques/physiologiques

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Organes cibles

Central nervous system, Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

53.6 °F

Point d'éclair (°C)

12 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Baumgart J, et al.
Biomaterials, 33(7), 2345-2350 (2012)
Human renal organic anion transporter 1-dependent uptake and toxicity of mercuric-thiol conjugates in Madin-Darby canine kidney cells
Aslamkhan AG, et al.
Molecular Pharmacology, 63(3), 590-596 (2003)
Dalia Chávez-García et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(6), 2396-2406 (2020-02-06)
Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
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