T9784

Tricine

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• Synonyme(s) : N-[Tris(hydroxyméthyl)méthyl]glycine
• Formule linéaire : (HOCH₂)₃CNHCH₂CO₂H
• Numéro CAS: 5704-04-1
• Poids moléculaire : 179.17
• Numéro Beilstein : 1937804
• Numéro CE : 227-193-6
• Numéro MDL: MFCD00004277
• Code UNSPSC : 12161700
• ID de substance PubChem : 24900603
• Nomenclature NACRES : NA.25

Propriétés

• Gamme de produits: BioXtra
• Pureté: ≥99% (titration)
• Forme: powder
• Impuretés: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• Résidus de calcination: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• Plage de pH utile: 7.4-8.8
• pKa (25 °C): 8.1
• Solubilité: H₂O: 1 M, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤0.1%; sulfate (SO₄^2-): ≤0.05%
• Traces de cations: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• Absorption: ≤0.05 at 280 in H₂O at 1 M; ≤0.1 at 260 in H₂O at 1 M
• Application(s): diagnostic assay manufacturing
• Chaîne SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Clé InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

Description générale

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Application

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection
• as a component of lactotroph feeding medium for culturing lactotrophs
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

Constituant de tampons destinés à la séparation de peptides de faible poids moléculaire.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)