SAB4200697
Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal
clone TH-16, purified from hybridoma cell culture
Synonyme(s) :
TH, TYH
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
TH-16, monoclonal
Forme
buffered aqueous solution
Espèces réactives
rat, dog, rabbit, human, sheep, guinea pig, mouse, monkey, bovine
Concentration
~1 mg/mL
Technique(s)
immunoblotting: 1-2 μg/mL using whole extract of rat PC-12 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotype
IgG1
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TH(7054)
Description générale
Monoclonal Anti-tyrosine hydroxylase (mouse IgG1 isotype) is derived from the hybridoma TH-16 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat tyrosine hydroxylase. This antibody recognizes an epitope located in the N-terminal region of human, monkey, bovine, sheep, rabbit, dog, guinea pig, mouse and rat tyrosine hydroxylase (TH).
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, BH4 and O2 as co-substrates, and Fe2+ as a cofactor.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, BH4 and O2 as co-substrates, and Fe2+ as a cofactor.
Immunogène
purified rat Tyrosine Hydroxylase
Actions biochimiques/physiologiques
Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter,that is, the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuro-psychiatric diseases such as schizophrenia and Parkinson′s disease (PD).
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.
Autres remarques
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40.
Haycock JW
The Journal of Biological Chemistry, 265, 11682-11691 (1990)
Role of N-terminus of tyrosine hydroxylase in the biosynthesis of catecholamines.
Nakashima A, et al.
Journal of neural transmission (Vienna, Austria : 1996), 116, 1355-1362 (2009)
Chun Chen et al.
NPJ Parkinson's disease, 7(1), 39-39 (2021-05-14)
Here we report the application of a mass spectrometry-based technology, imaging mass cytometry, to perform in-depth proteomic profiling of mitochondrial complexes in single neurons, using metal-conjugated antibodies to label post-mortem human midbrain sections. Mitochondrial dysfunction, particularly deficiency in complex I
Chang Chen et al.
Metabolic brain disease, 34(1), 203-212 (2018-11-15)
Parkinson's disease (PD) is a common chronic neurodegenerative disease and greatly affects the quality of PD patients' life. Current symptomatic treatment of PD is limited. There are no effective treatment and drugs that could radically cure PD. Increasing experimental evidence
The V81M variant of tyrosine hydroxylase is associated with more severe freezing of gait in Parkinson's disease.
Tekin I, et al.
Parkinsonism & Related Disorders, 86-90 (2016)
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