MTOX1092
MRP1 Knockout Caco-2 Cells
human male colorectal tissue (Source Disease: colon adenocarcinoma)
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About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81
Produits recommandés
product name
MRP1 Knockout Caco-2 Cells, one vial
Source biologique
human male colorectal tissue (Source Disease: colon adenocarcinoma)
Forme
liquid
Technique(s)
drug transporter assay: suitable
permeability assay: suitable
Application(s)
ADME/TOX
Température de stockage
−196°C
Informations sur le gène
human ... ABCC1(4363)
Description générale
The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC1 (MRP1) efflux transporter.
Application
The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Caractéristiques et avantages
The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
- A functional knockout of the MRP1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP1 substrate
- The vial format enables the MRP1 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
Informations légales
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
Articles
We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.
Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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