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MP0030

Sigma-Aldrich

LookOut® Mycoplasma Elimination Kit

Synonyme(s) :

Mycoplasma contamination treatment

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1 KIT
427,00 $

427,00 $

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1 KIT
427,00 $

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

427,00 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

Utilisation

 kit sufficient for 5 mL mycoplasma elimination

Niveau de qualité

200

Conditionnement

pkg of 1 kit

Conditions de stockage

protect from light

Technique(s)

PCR: suitable

Application(s)

detection
microbiology

Température de stockage

2-8°C

Catégories apparentées

Description générale

The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.

Application

The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.

Caractéristiques et avantages

  • Minimal cytotoxic properties of the reagents
  • Highly effective
  • Retention of normal cell characteristics

Adéquation

The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.

Informations légales

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number
0000356675
0000356675
SLCQ9058
SLCQ0037
SLCL8660

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)

Articles

Why Use Antibiotics in Cell Culture?

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

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Protocoles

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Questions

1–2 of 2 Questions  

  1. How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?

    1 answer

    1. The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.

      Helpful?

  2. What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?

    1 answer

    1. The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.

      Helpful?

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