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MOD50

Kit de modification de l′ADN Imprint®

For bisulfite DNA conversion & purification

Synonyme(s) :

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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1 kit
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1 010,00 $

A propos de cet article

UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54

1 010,00 $

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Quality Segment

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Preparation Note

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

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usage

sufficient for 50 reactions

usage

-

usage

-

usage

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

20-25°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif
  • T3566
    Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
    FDS

signalword

Danger

supp_hazards

EUH031

Classe de stockage

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

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Certificats d'analyse (COA)

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Questions

1–6 of 6 Questions  

  1. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    1 answer

    1. Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

      Helpful?

  4. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. This kit can be used for 50 reactions.

      Helpful?

  5. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    1 answer

    1. Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

      Helpful?

  6. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

      Helpful?

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