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MOD50

Sigma-Aldrich

Kit de modification de l'ADN Imprint®

For bisulfite DNA conversion & purification

Synonyme(s) :

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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1 KIT
946,00 $

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1 KIT
946,00 $

About This Item

Numéro CE :
231-548-0
Code UNSPSC :
41116158
Nomenclature NACRES :
NA.54

946,00 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Utilisation

sufficient for 50 reactions

Température de stockage

20-25°C

Catégories apparentées

Description générale

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.[1][2][3]

Caractéristiques et avantages

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Stockage et stabilité

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Informations légales

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalFDS

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H290,H302,H314,H412

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Risques supp

EUH031

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000333642
0000333642
SLCQ0447
SLCD4557
SLCD4559

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Elena Ivanova et al.
Clinical epigenetics, 12(1), 64-64 (2020-05-13)
Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and reprogramming. Here, we have extended
David E Condon et al.
BMC bioinformatics, 19(1), 31-31 (2018-02-07)
Identification of differentially methylated regions (DMRs) is the initial step towards the study of DNA methylation-mediated gene regulation. Previous approaches to call DMRs suffer from false prediction, use extreme resources, and/or require library installation and input conversion. We developed a
Nelly Olova et al.
Genome biology, 19(1), 33-33 (2018-03-17)
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such
Hwan Young Lee et al.
International journal of legal medicine, 126(1), 55-62 (2011-06-01)
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called
Priyadarshini Kachroo et al.
Epigenomics, 10(2), 133-147 (2018-01-16)
To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and
Afficher tous les articles scientifiques apparentés

Articles

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

Protocoles

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Contenu apparenté

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Questions

1–6 of 6 Questions  

  1. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

      Helpful?

  2. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

      Helpful?

  3. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. This kit can be used for 50 reactions.

      Helpful?

  4. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    1 answer

    1. Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

      Helpful?

  5. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    1 answer

    1. Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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