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M7653

Sigma-Aldrich

Medium 199

With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Synonyme(s) :

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Modification of the original Medium 199.Earle′s salts are replaced with Hanks′ salts. Hanks′ salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

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Description
Tarif

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Etienne de Coulon et al.
iScience, 24(2), 102041-102041 (2021-02-04)
A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing simultaneously in vivo like strain events. Here, we introduce a linear strain single-cell electrophysiology (LSSE) system that meets these requirements by delivering highly reproducible unidirectional
Yining Li et al.
The Journal of biological chemistry, 292(6), 2301-2314 (2016-12-21)
Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.
Benoît X E Desbiolles et al.
Microsystems & nanoengineering, 6, 67-67 (2021-09-28)
Volcano-shaped microelectrodes (nanovolcanoes) functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access. These nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion
Emilie Brûlé et al.
Journal of the Endocrine Society, 5(4), bvab023-bvab023 (2021-04-03)
Loss-of-function mutations in the X-linked immunoglobulin superfamily, member 1 (IGSF1) gene result in central hypothyroidism, often associated with macroorchidism. Testicular enlargement in these patients might be caused by increases in follicle-stimulating hormone (FSH) levels, as IGSF1 has been proposed to
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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Contenu apparenté

Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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