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L3397

Sigma-Aldrich

LB Broth (Luria low salt)

Powder microbial growth medium

Synonyme(s) :

Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)

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About This Item

Code UNSPSC :
41106200
Nomenclature NACRES :
NA.85

Qualité

for molecular biology

Niveau de qualité

200

Stérilité

non-sterile

Forme

powder

Composition

NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

Technique(s)

microbiological culture: suitable

pH

6.8-7.2(1.55% solution)

Adéquation

suitable for molecular biology
nonselective for Escherichia coli
nonselective for coliforms

Application(s)

food and beverages
microbiology

Température de stockage

room temp

Catégories apparentées

Description générale

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Application

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Caractéristiques et avantages

Luria′s low salt LB powder provides:
  • Standard formulation
  • A budget-friendly alternative to liquid
  • Easy scale-up using larger package sizes

Notes préparatoires

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Giorgia Manzo et al.
ACS infectious diseases, 7(8), 2310-2323 (2021-07-31)
Reliable antimicrobial susceptibility testing is essential in informing both clinical antibiotic therapy decisions and the development of new antibiotics. Mammalian cell culture media have been proposed as an alternative to bacteriological media, potentially representing some critical aspects of the infection
Michel Godin et al.
Nature methods, 7(5), 387-390 (2010-04-13)
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in
Difco Manual, 272-272 (1998)
Luria broth (LB) and Luria agar (LA) media and their uses protocol
MacWilliams MP and Liao MK
Journal of Separation Science (2006)
Alex Golberg et al.
AAPS PharmSciTech, 10(3), 881-886 (2009-07-03)
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem
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Protocoles

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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