B0631

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

≥90%

• Synonyme(s) : 5-BrdUTP
• Formule empirique (notation de Hill) : C₉H₁₄BrN₂O₁₄P₃
• Numéro CAS: 102212-99-7
• Poids moléculaire : 547.04
• Numéro MDL: MFCD00057405
• Code UNSPSC : 41106305
• ID de substance PubChem : 24891527
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: synthetic (organic)
• Essai: ≥90%
• Forme: powder
• Solubilité: water: 50 mg/mL, clear, colorless to faintly yellow
• Température de stockage: −20°C
• Chaîne SMILES: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• Clé InChI: UWZGQOWZUSBZMC-UHFFFAOYSA-N

Description

Description générale

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.[1]

Application

5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:

• in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells[2]
• to label cleaved DNA ends in apoptosis assay in avian tissue sections[3]
• for incorporation into mice prostates for immunohistochemistry studies[4]

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges