This product has not been specifically tested for suitability in SCSA and sperm DNA structure assays. However, there are a number of publications that have used this product for such applications. A thorough review of the literature is recommended. Please see the link below to review one such publication:
https://www.sciencedirect.com/science/article/pii/S0188440901003289
A6014
Acridine Orange hemi(zinc chloride) salt
For nucleic acid staining in cells or gels
Synonyme(s) :
3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
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10 G
128,00 $
25 G
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100 G
602,00 $
About This Item
Formule linéaire :
C17H20ClN3 · HCl · 1/2ZnCl2
Numéro CAS:
Poids moléculaire :
369.96
Numéro C.I. (Colour Index):
46005
Beilstein:
3734978
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.52
128,00 $
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Produits recommandés
Niveau de qualité
Gamme de produits
BioReagent
Forme
powder
Composition
Dye content, ~80%
Technique(s)
nucleic acid detection: suitable
Solubilité
ethanol: 2 mg/mL
4 mg/mL (2-methoxyethanol (EGME))
water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
Adéquation
suitable for flow cytometry
suitable for microscopy
Température de stockage
room temp
Chaîne SMILES
Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
InChI
1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
Clé InChI
RAHGLSRJKRXOSY-UHFFFAOYSA-L
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Description générale
Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.
Application
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Suitable for
- detection of nucleic acids separated by gel electrophoresis
- fluorescence and epifluorescence microscopy
- analysis of mitochondria and lysosomes by flow cytometry
- DNA staining in apoptosis studies
Caractéristiques et avantages
- 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
- differential staining of single- and double-stranded polynucleotides
Principe
Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.[1]
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Muta. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
G K McMaster et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)
We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The
H Baisch et al.
Cell proliferation, 32(5), 303-319 (2000-01-05)
Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of
Z Darzynkiewicz et al.
Cytometry, 13(8), 795-808 (1992-01-01)
The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA
B Mdzewski et al.
Archivum immunologiae et therapiae experimentalis, 32(6), 677-683 (1984-01-01)
The distribution of the fluorescent euchrysine-binding grains in the peripheral and bonmarrow stem cells was estimated in 28 cases of different types of acute leukemia verified according to Lòffler's cytochemical classification. The relationship between the pattern of fluorescent euchrysine-binding grains
F Durrieu et al.
Cytometry, 36(2), 140-149 (1999-11-30)
Some forms of chemoresistance in leukemia may start from failure of tumour cells to successfully undergo apoptosis and Bcl-2 may play a role in this defect. Therefore, we evaluated the Bcl-2 content and synthesis in relation with the apoptotic potential
Articles
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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Is product A6014, Acridine Orange hemi(zinc chloride) salt, used for sperm chromatin structure assay (SCSA) and sperm DNA structure assay?
1 answer-
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